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ANXIOLYTIC-LIKE EFFECTS OF SYRINGIC ACID: A BEHAVIORAL STUDY

Yıl 2022, Cilt: 85 Sayı: 3, 378 - 387, 06.07.2022
https://doi.org/10.26650/IUITFD.1071463

Öz

Objective: The current study investigated the possible anxiolytic-like effects of syringic acid (SA) administration against deltamethrin (DTM) exposure in rats subjected to behavioral tests.
Materials and Methods: Wistar albino male rats weighing 250- 270 g were randomly divided into four groups as control (0.5 mg/kg corn oil), DTM (1.28 mg/kg), SA (25 mg/kg) and DTM+SA groups (1.28 mg/kg DTM, 25 mg/kg SA). Anxiety-like behaviors were evaluated by an open field test and a marble-burying test (Noldus Ethovision System).
Results: The SA treatment revealed a significant effect on time spent in the inner zone (100.36±0.04 sec, F(3.36)=71.13, η2=0.877, p=0.0001, p<0.05), number of crossings in the center (21.42±1.23 (n), F(3.36)=13.13, η2=0.522, p=0.0056, p<0.05) and fecal scores (2.9±0.21(n), F(3.36)=51.51, η2=0.811, p<0.05) via the open field. SA treatment exhibited a significant difference in the marble test compared with the other groups (F(3.36)=77.64, η2=0.962, p<0.0001).
Conclusions: The findings of this study represent the first step toward understanding the anxiolytic effects of SA, and our results suggest that SA treatment may be beneficial for anxiety-related disorders. Therefore, SA should be evaluated in a new drug design to increase the understanding of underlying anxiety disorders, and DTM may be a good resource for modeling anxiety behavior in Wistar rats.

Destekleyen Kurum

NONE

Proje Numarası

NONE

Teşekkür

NONE

Kaynakça

  • 1. Steimer T. The biology of fear- and anxiety-related behaviors. Dialogues Clin Neurosci 2022;4(3):231-49. [CrossRef] google scholar
  • 2. Steimer T. Animal models of anxiety disorders in rats and mice: some conceptual issues. Dialogues Clin Neurosci 2022;13(4):495-506. [CrossRef] google scholar
  • 3. Radhakrishnan A, Gulia Kamalesh K. Categories of Wistar Rats Based on Anxiety Traits: A Study Using Factor and Cluster Method. Ann Neurosci 2018;25(4):234-40.4. [CrossRef] google scholar
  • 4. Lai C-W, Chang CH. Adaptive anxious states and down-regulation of dopamine activity under amygdala activation in rats. Behav Brain Res 2019;361:1-6. [CrossRef] google scholar
  • 5. Ho YJ, Eichendorff J, Schwarting RKW. Individual response profiles of male Wistar rats in animal models for anxiety and depression. Behav Brain Res 2002;136(1):1-12. [CrossRef] google scholar
  • 6. Njung’e Ku, Handley SL. Evaluation of marble-burying behavior as a model of anxiety. Pharmacol Biochem Behav 1991;38(1):63-7. [CrossRef] google scholar
  • 7. Belzung C. Chapter 4.11 Measuring rodent exploratory behavior. Handbook of Molecular-Genetic Techniques for Brain and Behavior Research. Techniques in the Behavioral and Neural Sciences. France: Elsevier 1999;739-745:13. [CrossRef] google scholar
  • 8. Russell PA. Relationships between Exploratory Behaviour and Fear: A Review. Br J Psychol 1973;64(3):417-33. [CrossRef] google scholar
  • 9. Pinel JP, Treit D. Burying as a defensive response in rats. J Comp Physiol Psychol 1978;92(4):708-12. [CrossRef] google scholar
  • 10. Whimbey AE, Denenberg VH. Two independent behavioral dimensions in open-field performance. J Comp Physiol Psychol 1967;63(3):500-4. [CrossRef] google scholar
  • 11. Matsushita M, Egashira N, Harada S, Okuno R, Mishima K, Iwasaki K, et al. Perospirone, a Novel Antipsychotic Drug, Inhibits Marble-Burying Behavior via 5-HT1A Receptor in Mice: Implications for Obsessive-Compulsive Disorder. J Pharmacol Sci 2005;99(2):154-9. [CrossRef] google scholar
  • 12. Egashira N, Harada S, Okuno R, Matsushita M, Nishimura R, Mishima K, et al. Involvement of the sigma1 receptor in inhibiting activity of fluvoxamine on marble-burying behavior: Comparison with paroxetine. Eur J Pharmacol 2007;563(1-3):149-54. [CrossRef] google scholar
  • 13. Srinivasulu C, Ramgopal M, Ramanjaneyulu G, Anuradha CM, Suresh Kumar C. Syringic acid (SA)-A Review of Its Occurrence, Biosynthesis, Pharmacological and Industrial Importance. Biomed Pharmacother 2018;108:547-57. [CrossRef] google scholar
  • 14. Ogut E, Armagan K, Gül Z. The role of syringic acid as a neuroprotective agent for neurodegenerative disorders and future expectations. Metab Brain Dis 2022;37(4):859-80. [CrossRef] google scholar
  • 15. Ogut E, Sekerci R, Akcay G, Yildirim FB, Derin N, Aslan M, et al. Protective effects of syringic acid on neurobehavioral deficits and hippocampal tissue damages induced by sub-chronic deltamethrin exposure. Neurotoxicol Teratol 2019;76:106839. [CrossRef] google scholar
  • 16. Habr SF, Macrini DJ, Florio JC, Bernardi MM. Repeated forced swim stress has additive effects in anxiety behavior and in cathecolamine levels of adult rats exposed to deltamethrin. Neurotoxicol Teratol 2014;46:57-61. [CrossRef] google scholar
  • 17. Güzelad Ö, Özkan A, Parlak H, Sinen O, Afşar E, Öğüt E, et al. Protective mechanism of Syringic acid in an experimental model of Parkinson’s disease. Metab Brain Dis 2021;36(5):1003-14. [CrossRef] google scholar
  • 18. Ogut E, Akcay G, Yildirim FB, Derin N, Aslan M. The influence of syringic acid treatment on total dopamine levels of the hippocampus and on cognitive behavioral skills. Int J Neurosci 2020:1-9. [CrossRef] google scholar
  • 19. Jin S, Zhao Y, Jiang Y, Wang Y, Li C, Zhang D, et al. Anxiety-like behaviour assessments of adolescent rats after repeated maternal separation during early life. Neuro Report 2018;29(8):643-9. [CrossRef] google scholar
  • 20. Kumar A. Evaluation of toxicological and behavioral symptoms on deltamethrin treated albino rats. MOJAP 2018;5(1):63-7. [CrossRef] google scholar
  • 21. Denenberg VH. Open-Field Behavior in the Rat: What Does It Mean? Ann N Y Acad Sci 1969;159(3):852-9. [CrossRef] google scholar
  • 22. Zanda MT, Fadda P, Antinori S, Di Chio M, Fratta W, Chiamulera C, et al. Methoxetamine affects brain processing involved in emotional response in rats. Br J Pharmacol 2017;174(19):3333-45. [CrossRef] google scholar
  • 23. Sahgal A. Behavioural neuroscience: a practical approach. (The Practical Approach Series, 129) Oxford. UK: Oxford University Press, 1993:2.244. google scholar
  • 24. Sprowles JLN, Hufgard JR, Gutierrez A, Bailey RA, Jablonski SA, Williams MT, et al. Perinatal exposure to the selective serotonin reuptake inhibitor citalopram alters spatial learning and memory, anxiety, depression, and startle in Sprague-Dawley rats. Int J Dev Neurosci 2016;54(1):39-52. [CrossRef] 32. google scholar
  • 25. Tsuji M, Miyagawa K, Takeuchi T, Takeda H. Pharmacological characterization and mechanisms of the novel antidepressive- and/or anxiolytic-like substances identified from Perillae Herba. JPN J PHARMACOL 2008;28(4):159-67. google scholar
  • 26. Dalmagro AP, Camargo A, Zeni ALB. Morus nigra and its major phenolic, syringic acid, have antidepressant-like and neuroprotective effects in mice. Metab Brain Dis 2017;32(6):1963-73. [CrossRef] google scholar
  • 27. Chowdhury MR, Chowdhury KH, Hanif NB. In silico evaluation of therapeutic potentials of Syringic acid against some selected diseases. Phytomedicine 2020;7(2):53-7. [CrossRef] google scholar
  • 28. Ferah Okkay I, Okkay U, Gundogdu OL, Bayram C, Mendil AS, Ertugrul MS, et al. Syringic acid protects against thioacetamide-induced hepatic encephalopathy: Behavioral, biochemical, and molecularevidence. Neurosci Lett 2022;769:136385. [CrossRef] google scholar
  • 29. Zhao Y, Dang M, Zhang W, Lei Y, Ramesh T, Priya Veeraraghavan V, et al. Neuroprotective effects of Syringic acid against aluminium chloride induced oxidative stress mediated neuroinflammation in rat model of Alzheimer’s disease. J Funct Foods 2020;71:104009. [CrossRef] google scholar
  • 30. Bhattacharya SK, Mitra SK. Anxiogenic activity of quinine--an experimental study in rodents. Indian J Exp Biol 1992;30(1):33-7. google scholar
  • 31. Ricci EL, Ferreira Jr V, Habr SF, Macrini DJ, Bernardi MM, Spinosa HdS. Evidencias neuroquımicase comportamentais do efeito ansiogenico da deltametrina em ratos. Braz J Vet Res Anim Sci 2013;50(1):33-42. [CrossRef] google scholar
  • 32. Hannell A, Marklund N. Structured evaluation of rodent behavioral tests used in drug discovery research. Front Behav Neurosci 2014;8:252. [CrossRef] google scholar

SİRİNJİK ASİDİN ANKSİYOLİTİK BENZERİ ETKİLERİ: DAVRANIŞ ÇALIŞMASI

Yıl 2022, Cilt: 85 Sayı: 3, 378 - 387, 06.07.2022
https://doi.org/10.26650/IUITFD.1071463

Öz

Amaç: Bu çalışma, davranış testlerine tabi tutulan sıçanlarda deltametrin (DTM) maruziyetine karşı sirinjik asit (SA) uygulamasının olası anksiyolitik benzeri etkilerini araştırmak için yapılmıştır.
Gereç ve Yöntem: 250-270 gr ağırlığındaki Wistar albino erkek sıçanlar randomize olarak kontrol (0.5 mg/kg mısır yağı), DTM (1,28 mg/kg), SA (25 mg/kg) ve DTM+SA (1,28 mg/kg DTM, 25 mg/kg SA) olmak üzere dört gruba ayrıldı. Anksiyete benzeri davranışlar açık alan testi ve misket gömme testi (Noldus Ethovi- sion System) ile değerlendirildi.
Bulgular: Sirinjik asit tedavisi ile açık alan testinde, iç kısımda geçirilen süre (100,36±0,04 sn, F(3.36)=71,13, η2=0,877, p=0,0001; p<0,05), merkezdeki geçiş sayısı (21,42±1,23 (n), F(3.36)=13,13, η2=0,522, p=0,0056; p<0,05) ve dışkı skorlarında (2,9±0.21 (n), F(3.36)=51,51, η2=0,811; p<0,05) anlamlı farklılık ortaya çıktı. SA tedavisi misket gömme testinde diğer gruplara göre anlamlı bir farklılık gösterdi (F(3.36)=77,64, η2=0,962; p<0,0001).
Sonuçlar: Bu çalışmanın bulguları SA’nın anksiyolitik etkileri üzerine ilk adımı temsil ediyor ve sonuçlarımız SA tedavisinin anksiyete ile ilişkili bozukluklar için faydalı olabileceğini gösteriyor. Bu nedenle SA, altta yatan anksiyete bozukluklarının anlaşılmasını artırmak için yeni bir ilaç tasarımında değerlendirilmelidir ve DTM, Wistar sıçanlarında anksiyete davranışını modellemek için iyi bir kaynak olabilir.

Proje Numarası

NONE

Kaynakça

  • 1. Steimer T. The biology of fear- and anxiety-related behaviors. Dialogues Clin Neurosci 2022;4(3):231-49. [CrossRef] google scholar
  • 2. Steimer T. Animal models of anxiety disorders in rats and mice: some conceptual issues. Dialogues Clin Neurosci 2022;13(4):495-506. [CrossRef] google scholar
  • 3. Radhakrishnan A, Gulia Kamalesh K. Categories of Wistar Rats Based on Anxiety Traits: A Study Using Factor and Cluster Method. Ann Neurosci 2018;25(4):234-40.4. [CrossRef] google scholar
  • 4. Lai C-W, Chang CH. Adaptive anxious states and down-regulation of dopamine activity under amygdala activation in rats. Behav Brain Res 2019;361:1-6. [CrossRef] google scholar
  • 5. Ho YJ, Eichendorff J, Schwarting RKW. Individual response profiles of male Wistar rats in animal models for anxiety and depression. Behav Brain Res 2002;136(1):1-12. [CrossRef] google scholar
  • 6. Njung’e Ku, Handley SL. Evaluation of marble-burying behavior as a model of anxiety. Pharmacol Biochem Behav 1991;38(1):63-7. [CrossRef] google scholar
  • 7. Belzung C. Chapter 4.11 Measuring rodent exploratory behavior. Handbook of Molecular-Genetic Techniques for Brain and Behavior Research. Techniques in the Behavioral and Neural Sciences. France: Elsevier 1999;739-745:13. [CrossRef] google scholar
  • 8. Russell PA. Relationships between Exploratory Behaviour and Fear: A Review. Br J Psychol 1973;64(3):417-33. [CrossRef] google scholar
  • 9. Pinel JP, Treit D. Burying as a defensive response in rats. J Comp Physiol Psychol 1978;92(4):708-12. [CrossRef] google scholar
  • 10. Whimbey AE, Denenberg VH. Two independent behavioral dimensions in open-field performance. J Comp Physiol Psychol 1967;63(3):500-4. [CrossRef] google scholar
  • 11. Matsushita M, Egashira N, Harada S, Okuno R, Mishima K, Iwasaki K, et al. Perospirone, a Novel Antipsychotic Drug, Inhibits Marble-Burying Behavior via 5-HT1A Receptor in Mice: Implications for Obsessive-Compulsive Disorder. J Pharmacol Sci 2005;99(2):154-9. [CrossRef] google scholar
  • 12. Egashira N, Harada S, Okuno R, Matsushita M, Nishimura R, Mishima K, et al. Involvement of the sigma1 receptor in inhibiting activity of fluvoxamine on marble-burying behavior: Comparison with paroxetine. Eur J Pharmacol 2007;563(1-3):149-54. [CrossRef] google scholar
  • 13. Srinivasulu C, Ramgopal M, Ramanjaneyulu G, Anuradha CM, Suresh Kumar C. Syringic acid (SA)-A Review of Its Occurrence, Biosynthesis, Pharmacological and Industrial Importance. Biomed Pharmacother 2018;108:547-57. [CrossRef] google scholar
  • 14. Ogut E, Armagan K, Gül Z. The role of syringic acid as a neuroprotective agent for neurodegenerative disorders and future expectations. Metab Brain Dis 2022;37(4):859-80. [CrossRef] google scholar
  • 15. Ogut E, Sekerci R, Akcay G, Yildirim FB, Derin N, Aslan M, et al. Protective effects of syringic acid on neurobehavioral deficits and hippocampal tissue damages induced by sub-chronic deltamethrin exposure. Neurotoxicol Teratol 2019;76:106839. [CrossRef] google scholar
  • 16. Habr SF, Macrini DJ, Florio JC, Bernardi MM. Repeated forced swim stress has additive effects in anxiety behavior and in cathecolamine levels of adult rats exposed to deltamethrin. Neurotoxicol Teratol 2014;46:57-61. [CrossRef] google scholar
  • 17. Güzelad Ö, Özkan A, Parlak H, Sinen O, Afşar E, Öğüt E, et al. Protective mechanism of Syringic acid in an experimental model of Parkinson’s disease. Metab Brain Dis 2021;36(5):1003-14. [CrossRef] google scholar
  • 18. Ogut E, Akcay G, Yildirim FB, Derin N, Aslan M. The influence of syringic acid treatment on total dopamine levels of the hippocampus and on cognitive behavioral skills. Int J Neurosci 2020:1-9. [CrossRef] google scholar
  • 19. Jin S, Zhao Y, Jiang Y, Wang Y, Li C, Zhang D, et al. Anxiety-like behaviour assessments of adolescent rats after repeated maternal separation during early life. Neuro Report 2018;29(8):643-9. [CrossRef] google scholar
  • 20. Kumar A. Evaluation of toxicological and behavioral symptoms on deltamethrin treated albino rats. MOJAP 2018;5(1):63-7. [CrossRef] google scholar
  • 21. Denenberg VH. Open-Field Behavior in the Rat: What Does It Mean? Ann N Y Acad Sci 1969;159(3):852-9. [CrossRef] google scholar
  • 22. Zanda MT, Fadda P, Antinori S, Di Chio M, Fratta W, Chiamulera C, et al. Methoxetamine affects brain processing involved in emotional response in rats. Br J Pharmacol 2017;174(19):3333-45. [CrossRef] google scholar
  • 23. Sahgal A. Behavioural neuroscience: a practical approach. (The Practical Approach Series, 129) Oxford. UK: Oxford University Press, 1993:2.244. google scholar
  • 24. Sprowles JLN, Hufgard JR, Gutierrez A, Bailey RA, Jablonski SA, Williams MT, et al. Perinatal exposure to the selective serotonin reuptake inhibitor citalopram alters spatial learning and memory, anxiety, depression, and startle in Sprague-Dawley rats. Int J Dev Neurosci 2016;54(1):39-52. [CrossRef] 32. google scholar
  • 25. Tsuji M, Miyagawa K, Takeuchi T, Takeda H. Pharmacological characterization and mechanisms of the novel antidepressive- and/or anxiolytic-like substances identified from Perillae Herba. JPN J PHARMACOL 2008;28(4):159-67. google scholar
  • 26. Dalmagro AP, Camargo A, Zeni ALB. Morus nigra and its major phenolic, syringic acid, have antidepressant-like and neuroprotective effects in mice. Metab Brain Dis 2017;32(6):1963-73. [CrossRef] google scholar
  • 27. Chowdhury MR, Chowdhury KH, Hanif NB. In silico evaluation of therapeutic potentials of Syringic acid against some selected diseases. Phytomedicine 2020;7(2):53-7. [CrossRef] google scholar
  • 28. Ferah Okkay I, Okkay U, Gundogdu OL, Bayram C, Mendil AS, Ertugrul MS, et al. Syringic acid protects against thioacetamide-induced hepatic encephalopathy: Behavioral, biochemical, and molecularevidence. Neurosci Lett 2022;769:136385. [CrossRef] google scholar
  • 29. Zhao Y, Dang M, Zhang W, Lei Y, Ramesh T, Priya Veeraraghavan V, et al. Neuroprotective effects of Syringic acid against aluminium chloride induced oxidative stress mediated neuroinflammation in rat model of Alzheimer’s disease. J Funct Foods 2020;71:104009. [CrossRef] google scholar
  • 30. Bhattacharya SK, Mitra SK. Anxiogenic activity of quinine--an experimental study in rodents. Indian J Exp Biol 1992;30(1):33-7. google scholar
  • 31. Ricci EL, Ferreira Jr V, Habr SF, Macrini DJ, Bernardi MM, Spinosa HdS. Evidencias neuroquımicase comportamentais do efeito ansiogenico da deltametrina em ratos. Braz J Vet Res Anim Sci 2013;50(1):33-42. [CrossRef] google scholar
  • 32. Hannell A, Marklund N. Structured evaluation of rodent behavioral tests used in drug discovery research. Front Behav Neurosci 2014;8:252. [CrossRef] google scholar
Toplam 32 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm ARAŞTIRMA
Yazarlar

Eren Ogut 0000-0003-2506-9883

Güven Akçay 0000-0003-3418-8825

Fatoş Belgin Yıldırım 0000-0002-1939-6936

Narin Derin 0000-0001-5521-8929

Proje Numarası NONE
Yayımlanma Tarihi 6 Temmuz 2022
Gönderilme Tarihi 10 Şubat 2022
Yayımlandığı Sayı Yıl 2022 Cilt: 85 Sayı: 3

Kaynak Göster

APA Ogut, E., Akçay, G., Yıldırım, F. B., Derin, N. (2022). ANXIOLYTIC-LIKE EFFECTS OF SYRINGIC ACID: A BEHAVIORAL STUDY. Journal of Istanbul Faculty of Medicine, 85(3), 378-387. https://doi.org/10.26650/IUITFD.1071463
AMA Ogut E, Akçay G, Yıldırım FB, Derin N. ANXIOLYTIC-LIKE EFFECTS OF SYRINGIC ACID: A BEHAVIORAL STUDY. İst Tıp Fak Derg. Temmuz 2022;85(3):378-387. doi:10.26650/IUITFD.1071463
Chicago Ogut, Eren, Güven Akçay, Fatoş Belgin Yıldırım, ve Narin Derin. “ANXIOLYTIC-LIKE EFFECTS OF SYRINGIC ACID: A BEHAVIORAL STUDY”. Journal of Istanbul Faculty of Medicine 85, sy. 3 (Temmuz 2022): 378-87. https://doi.org/10.26650/IUITFD.1071463.
EndNote Ogut E, Akçay G, Yıldırım FB, Derin N (01 Temmuz 2022) ANXIOLYTIC-LIKE EFFECTS OF SYRINGIC ACID: A BEHAVIORAL STUDY. Journal of Istanbul Faculty of Medicine 85 3 378–387.
IEEE E. Ogut, G. Akçay, F. B. Yıldırım, ve N. Derin, “ANXIOLYTIC-LIKE EFFECTS OF SYRINGIC ACID: A BEHAVIORAL STUDY”, İst Tıp Fak Derg, c. 85, sy. 3, ss. 378–387, 2022, doi: 10.26650/IUITFD.1071463.
ISNAD Ogut, Eren vd. “ANXIOLYTIC-LIKE EFFECTS OF SYRINGIC ACID: A BEHAVIORAL STUDY”. Journal of Istanbul Faculty of Medicine 85/3 (Temmuz 2022), 378-387. https://doi.org/10.26650/IUITFD.1071463.
JAMA Ogut E, Akçay G, Yıldırım FB, Derin N. ANXIOLYTIC-LIKE EFFECTS OF SYRINGIC ACID: A BEHAVIORAL STUDY. İst Tıp Fak Derg. 2022;85:378–387.
MLA Ogut, Eren vd. “ANXIOLYTIC-LIKE EFFECTS OF SYRINGIC ACID: A BEHAVIORAL STUDY”. Journal of Istanbul Faculty of Medicine, c. 85, sy. 3, 2022, ss. 378-87, doi:10.26650/IUITFD.1071463.
Vancouver Ogut E, Akçay G, Yıldırım FB, Derin N. ANXIOLYTIC-LIKE EFFECTS OF SYRINGIC ACID: A BEHAVIORAL STUDY. İst Tıp Fak Derg. 2022;85(3):378-87.

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